L. L. Lloyd
May 17, 1991
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0
Influential Citations
78
Citations
Quality indicators
Journal
Journal of Chromatography A
Abstract
Abstract Rigid macroporous copolymers of styrene and divinylbenzene have been developed which have the high physical/mechanical stability necessary to operate under high-performance liquid chromatographic conditions of pressure and flow-rate. The matrices can be produced in a range of porosities, 100, 300, 1000 and 4000 A each with the controlled uniform pore geometry and pore size distribution essential for high efficiency separations. The small pore—high surface area macroporous poly(styrene—divinylbenzene) copolymer, PLRP-S 100 A, can be used in the unmodified form for reversed-phase separations of small molecules or by increasing the pore size, PLRP-S 300 A or greater, for the analysis of biological macromolecules. The surface characteristics of the hydrophobic poly(styrene-divinylbenzene) matrix can be altered by derivatization or coating to produce ion exchange functionalities or a hydrophilic surface for gel filtration chromatography. The hydrophilic surface can then be further derivatized to be utilised as a base matrix for affinity chromatography.