S. Day, L. Birnbaumer
1980
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Quality indicators
Journal
Endocrinology
Abstract
Estradiol benzoate (E; 20 μg, sc) was given on diestrus day 1 to 4-day cycling rats to advance the ovulatory LH surge by 1 day. Estrogen treatment is known to result in the maintenance of two apparently functional sets of corpora lutea (CL). Previously, our laboratory has demonstrated that E treatment results in the maintenance of LH-stimulable adenylyl cyclase activity in the 4-day-old CL on the day of expected proestrus. We investigated whether the effect of E was mediated by PRL by using an eryocryptine analog CB-154 to inhibit PRL release. We measured LH- and isoproterenol (Iso)-stimulable adenylyl cyclase activity and progesterone levels in 4- and 1-day-old CL and progesterone levels in serum. Iso was used as a control hormone to monitor receptor-mediated regulation of adenylyl cyclase activity. Progesterone was measured by RIA to examine whether LH-stimulable adenylyl cyclase activity correlated with CL function. We found that E treatment resulted in increased LH-stimulable adenylyl cyclase activity...