R. Lamed, Y. Levin, M. Wilchek
Apr 28, 1973
Citations
0
Influential Citations
158
Citations
Quality indicators
Journal
Biochimica et biophysica acta
Abstract
Abstract An improved method for the preparation of agarose hydrazide for coupling of nucleotides and nucleosides is described. Adipic acid dihydrazide is coupled to the agarose by the cyanogen bromide procedure, giving in one step the required agarose hydrazide. Aldehyde of IO 4 − oxidized nucleotides di- and triphosphates are coupled to the resin by hydrazone formation. The application of NADP bound to Sepharose hydrazide for the adsorption of glucose-6-phosphate dehydrogenase is described.