Jing Yang, Lei Huang, Zhengyu Guo
Apr 1, 2016
Citations
0
Influential Citations
7
Citations
Journal
Journal of Luminescence
Abstract
Abstract A novel fluorescent compound 7-diethylamino-3-[4׳-N-((2-ethoxy)ethanol)methyl aminobenzoylhydrazone]methyl coumarin ( I ) was synthesized and employed as a fluorescent probe for detecting lipopolysaccharides (LPS). This coumarin-based probe exhibited high fluorescence in aqueous environment, which could be quenched in the presence of Cu 2+ ion due to the complexation between I and Cu 2+ . However, the fluorescence of I can be recovered upon addition of LPS, because the binding between LPS and Cu 2+ ion is stronger than that between I and Cu 2+ ion, which disassociates the complexation of I and Cu 2+ ion and releases the fluorescence of I . Therefore, this three-component sensing system can be used to detect lipopolysaccharides in a facile manner. In addition, biological imaging studies have demonstrated that the probe can be used to evaluate the biosorptive capacity for Cu 2+ ion in living bacteria.