D. Macmillan, Lubna Arham
Aug 11, 2004
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0
Influential Citations
31
Citations
Journal
Journal of the American Chemical Society
Abstract
Herein cyanogen bromide is employed for the efficient production of N-terminal cysteine containing protein fragments for expressed protein ligation (EPL) from polyhistidine-tagged precursors. We provide three examples of efficient CNBr cleavage of fragments of the glycoprotein erythropoietin that can be ligated with peptides or glycopeptide mimetics potentially giving rise to semisynthetic glycoprotein therapeutics.