Sindhu Mathew, P. Adlercreutz
Jan 4, 2012
Citations
1
Influential Citations
7
Citations
Journal
Carbohydrate polymers
Abstract
Dodecyl glucooligosides, a class of interesting non ionic surfactant molecules were synthesized by cyclodextrin glucanotransferase from Bacillus macerans using either α-cyclodextrin (α-CD) or soluble starch as glycosyl donor and dodecyl β-d-glucoside (C12G1) or dodecyl β-d-maltoside (C12G2) as acceptor substrates. The primary coupling products obtained in the respective reactions were identified as dodecyl glucoheptaoside and dodecyl maltooctaoside by mass spectrometry. Higher yields of coupling products were obtained using α-CD as donor, while more dispoportionation occurred with starch. Nearly 78% conversion of the acceptor substrate C12G1 into dodecyl glucooligosides could be achieved at 132μg/ml of CGTase in 20min, while 93% of C12G2 could be transformed into products at 17.6μg/ml of enzyme in 120min using soluble starch as donor substrate. For applications requiring pure compounds like C12G7, synthesis using α-CD is advantageous. However, for applications in which a mixture of elongated alkyl glycosides is needed, reactions employing starch are clearly competitive.