E. Aboutabl, M. Luckner
Dec 1, 1975
Citations
0
Influential Citations
20
Citations
Journal
Phytochemistry
Abstract
Abstract Cyclopeptine and dehydrocyclopeptine, intermediates of cyclopenin biosynthesis in Penicillium cyclopium , can be reversibly transformed by homogenates of this fungus which contain cyclopeptine dehydrogenase. The enzyme can be assayed spectrophotometrically in the system NAD(P) + / NAD(P)H or by linking to diaphorase/2,6-dichlorophenolindophenol. While X-Press and acetone treatments of the mycelium are the most suitable disruption methods for assaying the enzyme on an analytical scale, grinding with sand proved more suitable for preparative work. Part of the total enzyme activity in the hyphae as well as in the conidiospores, is found in the cell wall-protoplasmic membrane-fraction. The soluble portion of the enzyme was 98-fold enriched. Cyclopeptine dehydrogenase activity increased at the beginning of the alkaloid-production-phase, indicating that the enzyme is concerned in alkaloid metabolism.