F. Khachik, G. Beecher
1985
Citations
0
Influential Citations
11
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The application of decapreno-β-carotene as an internal standard in quantification of the hydrocarbon carotenoids extracted from raw carrots has been thoroughly examined. Decapreno-β-carotene is a C50 β-carotene that has most of the requirements of an internal standard and it can be commercially synthesized in high purity. An isocratic high-performance liquid chromatographic (HPLC) system has been developed that separated all-trans-α-carotene, all-trans-β-carotene, and its l5,l5′-cis-isomer from this internal standard. Quantitative determination of the hydrocarbon carotenoids in carrots by HPLC using the internal standard technique gave values for α- and β-carotene similar to those obtained from α- and β-carotene standards alone.