F. Khachik, G. Beecher
1985
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Journal
Journal of chromatography
Abstract
The application of decapreno-beta-carotene as an internal standard in quantification of the hydrocarbon carotenoids extracted from raw carrots has been thoroughly examined. Decapreno-beta-carotene is a C50 beta-carotene that has most of the requirements of an internal standard and it can be commercially synthesized in high purity. An isocratic high-performance liquid chromatographic (HPLC) system has been developed that separated all-trans-alpha-carotene, all-trans-beta-carotene, and its 15,15'-cis-isomer from this internal standard. Quantitative determination of the hydrocarbon carotenoids in carrots by HPLC using the internal standard technique gave values for alpha- and beta-carotene similar to those obtained from alpha- and beta-carotene standards alone.