E. de Frenne, J. Eberspächer, F. Lingens
Mar 1, 1973
Citations
0
Influential Citations
24
Citations
Journal
European journal of biochemistry
Abstract
1 From the culture medium of bacteria, which utilize 5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone (pyrazon) as sole source of carbon four metabolites could be isolated. These metabolites were identified as 5-amino-4-chloro-2-(2,3-cis-dihydroxycyclohexa-4, 6-diene-1-yl)-3(2H)-pyridazinone (compound I), as 2-(5-amino-4-chloro-3-oxo-2,3-dihydro-2-pyridazino)-cis,cis-muconic acid (compound II), as 2-pyrone-6-carboxylic acid (compound III) and as 5-amino-4-chloro-3(2H)-pyridazinone (compound IV). 2 Cell-free extracts of the pyrazon-degrading bacteria were shown to metabolize 2-hydroxy-muconic acid with a stoichiometric release of CO2. 3 A pathway for the bacterial degradation of 5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone is proposed.