P. Corvini, R. Vinken, G. Hommes
Feb 1, 2004
Citations
3
Influential Citations
55
Citations
Journal
Biodegradation
Abstract
The degradation of the 4(3',5'-dimethyl-3'-heptyl)-phenol (p353NP)nonylphenol isomer in cultures of Sphingomonas TTNP3 supplemented withthe technical mixture of nonylphenol was first assessed. Then the radioactive andnon-labelled form of these diastereomers were both synthesised. The radioactiveisomers were synthesised using [ring-U-14C]-labelled phenol and3,5-dimethyl-3-heptanol by Friedel and Crafts alkylation. The time-course ofdegradation was performed with and without 14C-p353NP; balancing of radioactivity was calculated from different soluble fractions (organic, aqueous), bacterial biomass, and 14CO2 evolved as mineralization product. Thenoticeable portion of 14C bound to biomass showed that atleast the aromatic ring of 14C-p353NP was degradedand served as energy source and probably as carbon source forbacterial growth. In addition, the appearance of 3,5-dimethyl-3-heptanol,the nonanol corresponding with the side-chain of p353NP,was demonstrated in the bacterial media, and its concentrationdetermined during the course of fermentation. Besides the parent14C-p353NP, no other radioactive compounds,i.e. metabolites of 14C-p353NP were detectedin the media.