L. L. Muller, T. Jacks
Feb 1, 1975
Citations
5
Influential Citations
322
Citations
Quality indicators
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Abstract
Acidified 2,2-dimethoxypropane was used to chemically dehydrate biologic tissues for examination in the electron microscope. The ultrastructural integrities of single-celled algae, plant tissues (cotyledon, root, leaf) and animal tissues (liver, pancreas, muscle, cartilage) were maintained. Our technique was simpler and quicker than physically exchanging water for organic solvents (e.g., acetone, ethanol) as generally performed in microscopy.