C. J. Tandler, S. Fiszer de Plazas
Nov 1, 1975
Citations
1
Influential Citations
10
Citations
Journal
Life sciences
Abstract
Abstract Tetrahydrofuran, an efficient total lipid solvent, was found to precipitate chloroform-methanol soluble proteins from CM (chloroform-methanol, 2:1) lipid extracts of bovine and rat white and grey matter. To total lipid extracts containing proteolipid proteins 4 volumes of tetrahydrofuran are added; the precipitate is centrifuged and washed once with the same solvent. The product contains all the protein and about 1 to 2% of the lipids of the original lipid extract. It is insoluble in CM; solubility in CM is restored by addition of acetic acid or of the lipids recovered from the tetrahydrofuran-soluble fraction It is also soluble in glacial acetic acid and this solution can be diluted either with CM or with distilled water.