P. Prajapati, V. Vaghela
Sep 20, 2013
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Journal
JPC – Journal of Planar Chromatography – Modern TLC
Abstract
A sensitive method based on high-performance thin-layer chromatography (HPTLC) by using principle of densitometry with UV detection at 7nmax 242 nm for quantitative estimation of major pharmaceutically active compound, ciclesonide, 2-[(1S,2S,4R,8S,9S,11S,12S,13R)-6-cyclohexyl-11-hydroxy-9,13-dimethyl-16-oxo-5,7-dioxapentacyclo[10.8.0.02,9.04,8.013,18]icosa-14,17-dien-8-yl]-2-oxoethyl-2-methylpropanoate, which persists as a newer antiasthmatic drug of glucocorticoid class, is described here. Chromatographic separation was performed by semiautomatic CAMAG HPTLC instrument. The method was validated according to the International Conference on Harmonization (ICH) guidelines, illustrating to be accurate (98.92–100.26%) and precise (CV = 0.43–0.67 for intra-day and CV = 0.58–1.45 for inter-day) within the corresponding linear range. Limit of detection (LOD) and limit of quantification (LOQ) of sample were found to be 100 ng/spot and 303 ng/spot, respectively.