I. Kubo, Qing-Xi Chen, K. Nihei
May 1, 2003
Citations
3
Influential Citations
123
Citations
Quality indicators
Journal
Food Chemistry
Abstract
Abstract Dodecyl (C12) gallate (3,4,5-trihydroxybenzoate) was found to inhibit the oxidation of l -3,4-dihydroxyphenylalanine ( l -DOPA) catalyzed by tyrosinase. This inhibition is a slow and reversible reaction with residual enzyme activity. The inhibition kinetics analyzed by Lineweaver–Burk plots revealed that dodecyl (lauryl) gallate is an uncompetitive inhibitor (KIS=0.636 mM). Gallic acid, the parent compound of dodecyl gallate, acts as a reducing agent for o-quinone produced by the enzymic oxidation.