Kanyi Pu, Liping Cai, B. Liu
Jul 10, 2009
Citations
0
Influential Citations
70
Citations
Quality indicators
Journal
Macromolecules
Abstract
Cationic poly[9,9-bis(6′-N,N,N-trimethylammonium)hexyl)fluorene-alt-4,7-(2,1,3-benzothiadiazole) dibromide] (PFBT) and poly[9,9-bis(6′-(N,N,N-trimethylammonium)hexyl)fluorenyldivinylene-alt-4,7-(2,1,3-benzothiadiazole) dibromide] (PFVBT) are designed and synthesized to serve as multicolor light-up probes for biomolecular quantification. Because of the charge-transfer electronic states of the polymers, they exhibit weak fluorescence in aqueous media which can be significantly enhanced by increasing the hydrophobicity of polymeric microenvironment. Molecular orbital simulations further reveal that the presence of vinyl bonds endows PFVBT with a stronger charge-transfer character relative to that of PFBT. Both PFBT and PFVBT exhibit linear fluorescence enhancement as a function of bovine serum albumin (BSA) or DNA concentration in buffer solution, allowing effective biomolecular quantification. Of particular interest is that the light-up responses of PFBT or PFVBT in the presence of BSA and DNA are different...