P. Krubasik, S. Takaichi, T. Maoka
Sep 1, 2001
Citations
5
Influential Citations
64
Citations
Journal
Archives of Microbiology
Abstract
Abstract. Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2′R,6′R)-decaprenoxanthin di-(β-D)-glucoside, but in three-fold higher amounts. In addition, decaprenoxanthin diglucoside fatty acid esters and the intermediates nonaprene, 2-(3-methyl-2-butenyl)-ε,ψ-carotene, and sarcinene, 2,2′-bis(3-methyl-2-butenyl)-ε,ε-carotene were identified as minor carotenoids. The pink mutants MV40 and MV60 synthesized only lycopene. From another pink mutant, MV70, novel C50-carotenoids were isolated. By NMR and mass spectroscopy, nonaflavuxanthin, 2-(4-hydroxy-3-methyl-2-butenyl)-1,16-didehydro-1,2-dihydro-ψ,ψ-carotene, and flavuxanthin, 2,2′-bis(4-hydroxy-3-methyl-2-butenyl)-1,16,1′,16′-tetradehydro-1,2,1′,2′-tetrahydro-ψ,ψ-carotene, were identified. The identification of these intermediates revealed the detailed pathway for the formation of decaprenoxanthin derivatives in Corynebacterium glutamicum.