J. Ferguson, Andrew G. Baxter, P. Young
Jan 24, 2005
Citations
4
Influential Citations
137
Citations
Quality indicators
Journal
Analytica Chimica Acta
Abstract
Immunochemical screening assays using surface plasmon resonance have been developed for chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, prawn and cows' milk using a sensor chip coated with a chloramphenicol derivative and an antibody. The antibody cross-reacted with chloramphenicol glucuronide 73.8% (poultry), 69.2% (honey), 75.7% (prawn) and 84.8% (milk). There was no cross-reaction with similar drugs or other commonly used antibiotics. The assay allowed the direct analysis of bovine milk (fat content ∼3.5%). Poultry, honey and prawn samples were extracted with ethyl acetate followed by analysis on the biosensor. The decision limits (CCa) for each assay were determined as: poultry (0.005 μg kg -1 ), honey (0.02 μg kg -1 ), prawn (0.04 μg kg -1 ) and milk (0.04 μg kg -1 ) and the detection capabilities (CCβ) were 0.02, 0.02, 0.07 and 0.05 μg kg -1 , respectively. Poultry muscle, honey and milk were spiked at 0.1 μg kg -1 and prawn at 0.15 μg kg -1 and the intra-assay precision (n = 10) calculated as 10.5, 5.0, 4.6 and 8.8%, respectively. Between run precision (n = 3) performed at the same levels yielded the following results: 3.0% (poultry), 4.7% (honey), 7.6% (milk) and 5.5% (prawn).