J. Wickramasinghe, W. Morozowich, W. E. Hamlin
Sep 1, 1973
Citations
0
Influential Citations
45
Citations
Journal
Journal of Pharmaceutical Sciences
Abstract
Abstract The pentafluorobenzyl ester of prostaglandin F 2α was synthesized on a preparative scale and was gas chromatographed as the tris(trimcthylsilyl) ether. The latter was found to be stable during GLC and highly sensitive to electron-capture detection. The lower limit of detection was 12.5 pg. of the ester, injected on- column as the silylated product. The nanogram scale conversion of prostaglandin F 2α to the ester, under conditions amenable to electron-capture GLC detection, was developed. The electron- capture GLC response was linear over the 0.03-0.84-ng. range of the ester, injected as the tris(trimethylsilyl) ether.