Song Han-mi
2014
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Journal
West China Journal of Pharmaceutical Sciences
Abstract
OBJECTIVE To establish an ion chromatography method for determination of 1,4- butanedisulfonate anion of ademetionine 1,4- butanedisulfonate. METHODS Dionex ICS 5000 ion chromatography was used. The Ionpac AS14 anion analytical column(250 mm × 4 mm) was used with the mixture of 3. 5 mmoL·L- 1sodium carbonate and 1. 0 mmoL·L- 1sodium hydrogen carbonate as the mobile phase. Conductivity detector was employed,and the flow rate was 2. 0 mL·min- 1,while the injection volume was 25 μL. RESULTS A good linearity for the content of 1,4- butanedisulfonate was found in the range of 0. 037- 558 μg·mL- 1(r =0.9996). The average recovery was99.9%(RSD =0.61%,n =9),and the minimum detection limit was0.3 ng. CONCLUSION The method could accurately detect 1,4- butanedisulfonate in ademetionine 1,4- butanedisulfonate,and can be used as an effective methed for the quality control of ademetionine 1,4- butanedisulfonate.