Y. Bazel, I. Hunka, Z. Kormosh
Dec 1, 2009
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Journal
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Abstract
A new sensitive and selective spectrophotometric method has been developed for the determination of [2-(2,6-dichloro-phenylamino)-phenyl]-acetic acid in pharmaceuticals in the presence of nicotinic acid. The method is based on the reaction of [2-(2,6-dichloro-phenylamino)-phenyl]-acetic acid with 1,3,3-trimethyl-5-phenyl-2-[3-(1,3,3-trimethyl-1,3-dihydro-indol-2-ylidene)-propenyl]-3H-indolium chloride (PIC) followed by the extraction of the formed ion associate into toluene and spectrophotometric detection at 581 nm. Appropriate experimental conditions were found to be pH 7.8-9.8 and 3.6x10(-4) mol L(-1) of PIC. The molar absorptivity is 5.0x10(-4) L mol(-1) cm(-1). The absorbance obeys Beer's law in the range 0.61-12.60 microg mL(-1) of [2-(2,6-dichloro-phenylamino)-phenyl]-acetic acid, and the detection limit calculated from a blank test was 0.20 microg mL(-1).