Shi Nan
2012
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Journal
Food Science and Technology International
Abstract
A high performance liquid chromatography-ultraviolet spectrometry(HPLC-UV) method has been developed for determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth.2-amino-6-methylpyridine and other relative impurities was achieved on a Alltima C18(250 mm×4.6 mm i.d.,5 μm) by using methanol-water-1% aqueous acetic acid solution(85:10:5,v/v/v) as the mobile phase at a flow rate of 1.0 mL/min.Detection was operated by UV absorption at a wavelength of 231 nm.The calibration curves were showed good linearity(r 2 =0.9994) within the concentration range of 5.0~500 mg/L for 2-amino-6-methylpyridine.The average recoveries were ranged from 99.76%~101.4%.And the standard deviation was less than 2.17%.The method was simple and accurate for the determination of 2-amino-6-methylpyridine in Blakeslea trispora fermentation broth.