Huang Cuil
2015
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Journal
China Oils and Fats
Abstract
A gas chromatography- mass spectrometry( GC- MS) analysis method was developed for determination of fatty acid esters of 3- chloro- 1,2- propane diol( 3- MCPD esters) in edible vegetable oil employing area ratio method for quantitative measurement. Samples were dissolved in methyl tert- butyl ether after addition of internal standard d5- 3- chloro- 1,2- dipalmitate. Then the samples were transesterified with sodium methoxide methanol solution for 4 min and the reaction was terminated by acidic sodium bromide. The transesterification products had a derivatization reaction with 0. 2 m L phenylboric acid at 45 ℃ for 20 min after liquid- liquid extraction,then the specimens were determined by GC- MS. The results showed that the method had the items of high sensitiveness and simple operation,and the limit of detection was 10 μg/kg. The effects of refining process and freezing storage time of different vegetable oils on the content of 3- MCPD esters were investigated. The results demonstrated that the generation of3- MCPD esters mainly came from deodorization section,but it had a decrease with the storage time prolonging.