B. Pavithra, K. Jayakumar, A. Bhat
2011
Citations
0
Influential Citations
4
Citations
Journal
Chronicles of Young Scientists
Abstract
Aim: The study aims to develop a simple, rapid and economical normal phase thin layer chromatography (TLC) method for identification and quantification of 5,7-dichloroquinolin-8-ol and 5-chloroquinolin-8-ol, two active ingredients of halquinol, an antimicrobial agent. Materials and Methods: The analysis was performed on silica gel 60 TLC plates pre-washed with disodium ethylenedinitrilotetraacetic acid disodium salt (Na 2 EDTA) solution with methanol-ethyl acetate-iso-propyl alcohol-ammonia solution [8:20:1:0.6 (v/v)] as the mobile phase. Detection and quantification was performed densitometrically at 247 nm. Results: Responses of both 5,7-dichloroquinolin-8-ol and 5-chloroquinolin-8-ol were linear functions of concentration in the range of 300-800 ng. The intraday precision and intermediate precision of the method for 5,7-dichloroquinolin-8-ol and 5-chloroquinolin-8-ol was determined and it was found to be precise. Conclusion: The TLC method developed for simultaneous quantitative determination of 5,7-dichloroquinolin-8-ol and 5-chloroquinolin-8-ol was found to be simple and economical. Therefore, it can be used in routine quality control analysis of halquinol in bulk drug powder and halquinol bolus.