M. Ikeda, H. Hattori, Y. Koyama
1984
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0
Influential Citations
3
Citations
Quality indicators
Journal
Journal of chromatography
Abstract
Gas chromatography with electron-capture detection was used to quantify the glucuronic acid conjugate of trichloroethanol, urochloralic acid. The conjugate was extracted into ethyl acetate--ethanol (19:1, v/v) under acidic conditions, and analyzed as its trifluoroacetylated methyl ester or as its acetylated methyl ester. As little as 0.2 microgram of the conjugate was quantified, and the method was applicable to urine, plasma and liver. The synthesis of urochloralic acid from trichloroethanol by rat liver microsomal fraction was demonstrated in the presence of uridine diphosphoglucuronic acid as coenzyme.