J. D. de Silva, N. Strojny, M. Brooks
Dec 1, 1974
Citations
0
Influential Citations
10
Citations
Journal
Analytica chimica acta
Abstract
Abstract A sensitive and specific fluorimetric assay was developed for the determination of (d,l)-6-chloro-α-methyl-carbazole-2-acetic acid [I] in blood and urine, based on the intrinsic fluorescence of the compound in 1%, acetic acid in ethanol. The compound can also be determined by gas chromatography based on the response of its methyl ester to electron-capture detection. A t.l.c. step is used in the assay, not only to ensure specificity, but also as a necessary clean-up step. The fluorimetric method was applied to the determination of blood levels and the urinary excretion of the compound in a dog after intravenous administration of a single 10 mg kg-1 dose.