H. Ying
2000
Citations
0
Influential Citations
1
Citations
Journal
West China Journal of Pharmaceutcal Sciences
Abstract
OBJECTIVE:To establish a HPLC method for the determination of 3 methylflavone 8 carboxylic acid(MFCA),the main metabolite of flavoxate,in human plasma.METHODS:MFCA and the internal standard were extracted with ethyl acetate after alkali hydrolysis. The mobile phase consisted of methanol and 20 mmol·L -1 potassium dihydrogen phosphate(70∶30).The eluate was monitored at 310 nm.RESULTS:The calibration curve was linear within the range of 0.25~32 μg·ml 1 .The recoveries of extraction were 96.1%~100.3%.The recoveries of methodology were 96.8%~104.0%.Within day RSD ranged from 0.52% to 2.30%.Between day RSD ranged from 1.39% to 2.97%.CONCLUSION:This method is accurate and reliable.It has been applied successfully to the determination of MFCA in human plasma for the pharmacokinetic study of flavoxate.