L. Puignou, J. Casal, F. Santos
May 9, 1997
Citations
0
Influential Citations
34
Citations
Journal
Journal of Chromatography A
Abstract
Abstract The use of capillary electrophoresis (CE) for the determination of heterocyclic aromatic amines in a complex matrix is reported. This work focuses on the development of a suitable procedure for the quantitation of 2-amino-3,4-dimethylimidazo [4,5- f ]quinoline (MeIQ), 2-amino-3,8-dimethylimidazo[4,5- f ]quinoxaline (MeIQ x ), 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine, 3-amino-1-methyl-5H-pyrido[4,3- b ]indole and 2-amino-6-methyldipyrido[1,2- a :3′,2′- d ]imidazole at ppb level in a commercial meat extract. The influence of pH and buffer concentration, applied potential, temperature and injection mode were investigated. Several solid-phase extraction steps were performed for the clean-up of the sample, and a 10 m M KCl–HCl (pH 2.20) solution was used as running electrolyte for the separation by CE. Furthermore, figures of merit of the method were calculated, and detection limits ranging from 25–50 ng ml −1 were obtained. MeIQ and MeIQx were detected in the meat extract at levels of 9.3 ng g −1 and 10.4 ng g −1 , respectively. The analytical results were verified by a high-performance liquid chromatography method previously established.