Peng Zhi-bing
2006
Citations
0
Influential Citations
0
Citations
Journal
Journal of Nanchang University
Abstract
A novel and sensitive method for the determination of benproperine phosphate in oral solution has been established using capillary electrophoresis(CE) coupled with electrogenerated-chemiluminescence(ECL) detection,based on the ECL reaction of tris(2,2'-bipyridyl) ruthenium(Ⅱ) with the tertiary amino groups of the benproperine analyte.Electrophoresis was performed using an uncoated silica capillary(30 cm×25 μm).10 mM sodium phosphate buffer(pH=8.0) was used as the running buffer and the separation voltage was 18 kV.The solution in the detection cell was 50 mM sodium phosphate(pH=8.0) and 5 mM Ru(bpy)~(2+)_3.Benproperine phosphate in the sample could be separated and detected with in 100 s.The linear concentration of benproperine phosphate ranged from 10~(-7) to 10~(-5) mol/L(with a correlation coefficient of 0.999 6).The limit of detection was 10~(-8) mol/L(S/N=3).This method is simple and quick in operation,sensitive and reliable in determination results and it can be used for the quality control of benproperine phosphate.