T. Einig, W. Dehnen
Apr 21, 1995
Citations
1
Influential Citations
35
Citations
Journal
Journal of chromatography. A
Abstract
A method was developed for the determination of the specific benzene metabolite S-phenylmercapturic acid in urine. The analyte is determined by HPLC with fluorescence detection after solid-phase extraction of urine with C18 material and hydrolysis followed by precolumn derivatization. The samples are separate by a column-switching method with a dual column system. As the method is highly sensitive (detection limit ca. 1 microgram/l), urinary S-phenylmercapturic acid concentrations for non-exposed persons (e.g., non-smokers) can also be measured precisely.