Chuannan Luo, Xueying Wang, Zhongpeng Xu
Jun 11, 2009
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2009 3rd International Conference on Bioinformatics and Biomedical Engineering
Abstract
Sulfobutyl-β-cyclodextrin could remarkably decrease the fluorescence intensity of Neutral Red. In contrast, fluorescence intensity of Neutral Red enhanced efficiently when benzidine was presence. Consequently, based on the competitive interaction between benzidine and Neutral Red, a new fluorescence spectrographic method of the determination of benzidine was established using sulfobutyl-β-cyclodextrin and Neutral Red as a supermolecule probe in B-R buffer solution (pH 2.75). The linear range was from 2.00×10 mol/L to 1.80×10 mol/L, the limit of detection was 2.61×10 mol/L. It is indicated that this method had high sensitivity, good selectivity and convenient application. This method had been applied to the determination of benzidine in industry waste water. A preliminary discussion was also given on the mechanism of the fluorescence reaction system. Keywords-Sulfobutyl-β-cyclodextrit; Neutral Red; Benzidine; supermolecular probe