N. Othman, S. A. Omer
Mar 1, 2009
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Journal
Journal of Education Science
Abstract
A spectrophotometric method for the assay of trace amounts of bromhexine-HCl based on the reaction of bromhexine-HCl with nitrite ion to form the corresponding diazonium salt followed by coupling reaction with pyrogallol in a acidic medium to form a stable and a soluble yellow azo dye with maximum absorption at 387 nm. Beer’s law is obeyed in the concentration range of 20-280g of bromhexine-HCl in a final volume of 25 ml (0.811.2 ppm) with a molar absorptivity of 1.03110 4 L.mol -1 .cm -1 , a relative error of -0.36 to +0.72 % and a relative standard deviation of 1.07 to 1.72 %, depending on the concentration level of bromhexine-HCl. The method has been Spectrophotometric Determination of Bromhexine Hydrochloride ... 20 successfully applied for the assay of bromhexine-HCl in various pharmaceutical preparations. INTRODUCTION Bromhexine is used for respiratory infections, such as the cold and influenza [1]. Bromhexine hydrochloride, N-(2-amino-3,5dibromophenylmethyl)-N-methyl-cyclohexylamine hydrochloride has the following structure[2]: Bromhexine hydrochloride Several spectrophotometric methods have been reported for the estimation of bromhexine–HCl using different reagents such as, rose Bengal in sulphuric acid medium [3], citric acid-AC2O in chloroform medium [4], p-dimethylamino benzaldehyde in highly acidic medium [5], bromocresol purple [6], tropaeoline, naphthalene blue 12Br and azocarmon Greact acidic dyes in non aqueous medium (chloroform)[7], p-dimethylaminobenzaldehyde in the presence of sodium dodecyl sulphate[8], after diazotisation, the corresponding bromhexin diazonium salt is coupled with a suitable agent such as, N-(1-naphthyl) ethylenediamine[9], 1-naphtylamine [10], resorcinol [11], 2-naphtho [12], orcinol [13]. Also, derivative and mults wavelength [14] and first derivative spectrophotometric methods [15] have been used in determination bromhexine-HCl. However, some of these proced-ure suffer from one or another disadvantage such as poor sensitivity [5] or require nonaqueous medium [4,7,14]. The objective of the investigation reported in this paper is to evaluate a simple spectrophotometric method for the determination of bromhexine-HCl. The method involves the diazotisation of bromhexineHCl and subsequent coupling with pyrogallol reagent as a coupling agent to form a highly coloured dye. that has been proved successfully for the assay of bromhexine-HCl in its different pharmaceutical preparations. Experimental Instruments C N CH3 . HCl H