M. Paz, O. Blumenfeld, M. Rojkind
Mar 1, 1965
Citations
2
Influential Citations
180
Citations
Quality indicators
Journal
Archives of biochemistry and biophysics
Abstract
Abstract N -Methyl benzothiazolone hydrazone reacts with various carbonyl compounds. The azine and “osazine” derivatives formed under defined conditions have characteristic spectra which permit the identification of saturated and unsaturated aldehydes and ketones, keto acids, and many other related compounds in a simple spectrophotometric assay. The azines of aldehydes can be further reacted with the oxidized form of MBTH to give rise to tetraazopentamethine cyanine dyes used in a colorimetric procedure. The sensitivity of both methods is such that microgram amounts of a carbonyl compound can be identified and measured rapidly and accurately. Since carbohydrates having pyranose structures are unreactive in the colorimetric and spectrophotometric methods, many aldehydes and ketones can be measured in their presence.