M. Sorouraddin, J. Manzoori, E. Kargarzadeh
Dec 1, 1998
Citations
0
Influential Citations
94
Citations
Quality indicators
Journal
Journal of pharmaceutical and biomedical analysis
Abstract
A novel spectrophotometric method is described for the determination of epinephrine (EP) and norepinephrine (NE). The method is based on the development of a red colour with sodium bismuthate, as a sensitive chromogenic reagent, in aqueous medium at pH 3. Oxidation of these catecholamines produces aminochrome derivatives which can be measured spectrophotometrically at 486.0 nm. Calibration graphs are linear in the range 4.8-800 (micromol l(-1)) for epinephrine bitartarate and 4.8-600 (micromol l(-1)) for norepinephrine bitartarate with detection limits of 0.26 (micromol l(-1)) and 2.46 (micromol l(-1)) for epinephrine and norepinephrine bitartarate salts, respectively. The method has successfully been applied to determination of these catecholamines in pharmaceutical preparations.