K. Fossdal, E. Jacobsen
Aug 1, 1971
Citations
0
Influential Citations
18
Citations
Journal
Analytica chimica acta
Abstract
Abstract The electroreduction of chloramphenicol has been studied by polarography, cyclic voltammetry, chronopotentiometry and coulometry. The experimental results lead to the conclusion that the drug undergoes a slow 2-electron reduction of the nitro group which is followed by a fast 2-electron reduction to hydroxylamine. At more negative potentials the hydroxylamine group is further reduced to the amine. In acetate buffer pH 4.7 with 0.003 % decylamine present, the drug produces a very well-defined, diffusion-controlled 4-electron polarographic wave. The current is proportional to the concentration and permits the drug to be determined by polarography in the concentration range 0.3–600 μg ml -1 . A rapid polarographic method for the determination of chloramphenicol in milk is proposed.