Yingju Liu, Wuyi Zhou
Jul 1, 2006
Citations
0
Influential Citations
26
Citations
Journal
Analytical Sciences
Abstract
Tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence (ECL) detection in a capillary electrophoresis separation system was used for the determination of chlorpheniramine (CPM). The experimental conditions, such as the applied potential, separation voltage, injection voltage, injection time and the pH of the separation buffer were considered in detail. The ECL intensity showed two linear responses to CPM, i.e. , from 15 μM to 1 mM and from 0.8 μM to 15 μM with a detection limit of 0.5 μM. The binding of CPM with human serum albumin was also monitored using this method and the binding constant was estimated to be 4.1 × 10^3 M^–1.