Liu De-kun
2011
Citations
0
Influential Citations
3
Citations
Journal
The Chinese Pharmaceutical Journal
Abstract
OBJECTIVE To determine the contents of methylophiopogonanone A,methylophiopogonanone B and 6-aldehydo-isoophiopogonone A in Radix Ophiopogonis.METHODS Waters Symmetry C18 column(4.6 mm×250 mm,5 μm) was used with phosphoric acid(0.05%,v/v) and acetonitrile as the mobile phase.Stepwise gradient elution was adopted.The flow rate was 1.0 mL·min-1,the detection wavelength was 296 nm,and the column temperature was maintained at 30 ℃.RESULTS The calibration curves showed good linearity within the range of 9.68-96.8,4.96-49.6 and 4.18-41.8 μg·mL-1 for methylophiopogonanone A,methylophiopogonanone B and 6-aldehydo-isoophiopogonone A,respectively.The mean recoveries(n=6) were 100.1%(RSD 1.03%),99.5%(RSD 1.35%) and 100.2%(RSD 1.16%),respectively.CONCLUSION This method is simple and accurate.And it can be used for the determination of methylophiopogonanone A,methylophiopogonanone B and 6-aldehydo-isoophiopogonone A in Radix Ophiopogonis.