Sheida Sharifi, H. Michaelis, E. Lotterer
Apr 1, 1993
Citations
0
Influential Citations
7
Citations
Journal
Journal of Liquid Chromatography & Related Technologies
Abstract
Abstract A selective and sensitive method for the determination of coumarin and its main metabolites 7-hydroxycoumarin, 7-hydroxycoumarin-glucuronide and 3-hydroxycoumarin in human plasma and/or urine is described. Coumarin and 7-hydroxycoumarin were extracted from plasma with n-hexane/chloroform and with chloroform. After evaporation under vacuum, the residue was redissolved in methanol/water and injected onto the HPLC column (LiChroCART 250-4, RP 8e 5 μm; Merck, Darmstadt, Germany). The mobile phase consisted of methanol/water/tetrahydrofuran/acetic acid (45:A0:10:5). 7-Hydroxycoumarin-qlucuronide in plasma was enzymatically converted to 7-hydroxycoumarin by incubation with a mixture of β-glucuronidase and sulphatase in citrate-hydrochloric acid-buffer. Analysis of 7-hydroxycoumarin and its glucuronide in Correspondence Address: Dr. med. Sheida Sharifi. University of Gottingen, Robert-Koch-Strasse 40, D-3400 Gottingen, Germany urine was performed without extraction using a linear gradient system of meth...