F. Nachtmann, K. W. Budna
Jun 11, 1977
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0
Influential Citations
52
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Journal
Journal of Chromatography A
Abstract
Abstract The separation and determination of the 4-nitrobenzoates of some monosaccharides, disaccharides and trisaccharides by high-performance liquid chromatography are described. The relatively apolar derivatives permit rapid isocratic separations on polar adsorbents such as silica gel and alumina. The sugar derivatives are detectable with high sensitivity, detection limits being in the nanogram region for all sugars tested. The extinction maximum of 260 nm permits the use of low-cost UV detectors (254 nm). A complete analysis takes 70–75 min, 10–15 min of this time-being needed for the chromatographic separation. The practical aspects of the method are demonstrated by some examples.