Zhang Xian-cheng
2004
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Journal
West China Journal of Pharmaceutical Sciences
Abstract
OBJECTIVE To establish an HPLC method applicable to the analysis of dicloxacillin sodium in the presence of its degradants.METHODS Peak homogeneity of the resolving drug peak was assessed by the shape of the derivative spectroscopy.Shimadzu ODS column(200 mm×4.6mm,5μm) was used.The mobile phase was methanol-0.02 mol·L -1 monobasic potassium phosphate (55∶45,added 0.01 mol·L -1 tetramethylammonium chloride,adjust to pH 5.0) and the flow rate was 1.0 ml·min -1.The UV detective wavelength was 225 nm.RESULTS The calibration curve was linear in the range of 30-600 μg·ml -1 (r=0.9998).The average recovery was 100.37% with RSD of 0.52%.The method precision was 0.37%(n=6).CONCLUSION The method is selective,simple and accurate.It is applicable to the analysis of dicloxacillin sodium in the presence of its degradants.