S. Nene, B. Anjaneyulu, T. G. Rajagopalan
Apr 1, 1998
Citations
0
Influential Citations
6
Citations
Journal
Journal of Chromatography A
Abstract
Abstract Diethylcarbamazine, 1-diethylcarbamyl-4-methylpiperazine, (DEC), is an antifilarial drug showing good microfilaricidal activity. Pharmacokinetic studies on this drug also are few probably due to lack of sufficient sensitivity of the methods reported in the literature for the determination of DEC concentration in biological fluids, viz. spectrophotometric methods [1–3] and the more recent improved gas chromatographic (GC) methods [4, 5]. The present report describes a GC procedure using an alkali flame ionization detector, for the quantitative determination of DEC in blood samples. The method can be used for measuring 50 ng of DEC (free base) and above in blood with a good degree of precision and accuracy. 1-Diethylcarbamyl-4-ethylpiperazine was used as the internal standard in the anayytical procedure.