Margarita Lopez, M. Alvarez, A.J.Miranda Ordieres
Jan 19, 1996
Citations
0
Influential Citations
37
Citations
Journal
Journal of Chromatography A
Abstract
A reversed-phase liquid chromatographic method involving precolumn derivatization with 9-fluorenyl-methylchloroformate (FMOC) has been developed for the determination of dimethylamine (DMA) in groundwater. FMOC reacts rapidly with DMA under mild conditions, and the derivative is separated from matrix components and FMOC degradation products on a C18 column using isocratic elution with a mobile phase of 0.03 M acetate buffer (pH 4.0)-acetonitrile (30:70, v/v). Mean recovery was 98.5±4% and a detection limit of 4.5·10−7 M, corresponding to an injected amount of 3.6 pmol, was estimated using fluorimetric detection with excitation and emission wavelengths of 265 and 310 nm, respectively. No matrix interferences were found even when highly coloured and/or cloudy samples were examined. Several mixtures containing eleven amines, diamines and amino acids can be resolved using this method.