Di Dong-hua
2009
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Journal
Journal of Shenyang Pharmaceutical University
Abstract
Objective To develop a sensitive and specific liquid chromatography-tandem mass spectrometry(LC-MS-MS)method for determination of erythromycylamine in human plasma.Methods Erythromycylamine was extracted by diethyl ether and dichloromethane from human plasma,and then separated on a C18 column.The mobile phase consisted of methanol-water-formic acid(V∶V∶V=80∶20∶0.5)and the flow rate was 0.6 mL·min-1.A mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode.In multiple reaction monitoring(MRM)mode,the ion combination of m/z 749→m/z 591+ and m/z 748→m/z 158+ were used to quality erythromycylamine and an internal standard(clarithromycin),respectively.Results Chromatograms showed no endogenous interfering peaks in the respective blank human plasma samples.Each analysis was completed within 4 min.The calibration was linear in the concentration range within 1.0-1 000 μg·L-1 and quantitative limit was 1.0 μg·L-1.The intra-batch and inter-batch RSD were less than 15%.Conclusions The method is proved to be suitable for clinical investigation of erythromycylamine pharmacokinetics,which offers advantages of specificity,quickness and higher sensitivity over the previously reported methods.