Zhang Xiao
2002
Citations
0
Influential Citations
0
Citations
Journal
Chinese Journal of Pharmaceutical Analysis
Abstract
Objective: To establish a RP - HPLC method to determine N - glycyl - L - glutamine monohydrate (GGM)with 2,4 -dinitrofluorobenzene(DNFB)as a precolumn derivative regent. Methods:2,4 - Dinitrophenyl -N - glycyl - L - glutamine monohydrate ( DNP - GGM ) was made from the mixture of DNFB with GGM on the alkaline condition at 80℃. Then the reaction solution was analyzed by Shimadzu SCL - 10AVP HPLC instrument on ODS column with a flow phase of acetonitrile - aqueous solution of 0 . 05 mol @@L-1 sodium acetate , pH 6 . 4 (20: 80). Detection wavelength was 360 nm. Flow rate was 1. 0 mL·min-1. Results:The derivative DNP - GGM could be separated from 2,4 - dinitrophenol which was the hydrolysate of DNFB on an ODS column within 10 minutes. There was a good linear correlation in the range of 16- 80μg·mL-1, r = 0. 994 3 , Y = 1. 243 6×10~(-4)X, RSD =0. 31%(n=5). Conclusion: This method was the best assay to determine GGM.