Min Zhu, Xue-mei Huang, Liu-Zhan Liu
Aug 1, 1997
Citations
1
Influential Citations
28
Citations
Journal
Talanta
Abstract
A new hydrogen donor for peroxidase, Eriochrome black T, was reported for the first time. Steady-state catalytic velocity depends upon enzyme and substrate concentrations, and a Michaelis-Menten K(m) value of 1.72x10(-5) mol l(-1) and a V(max) value of 4.43x10(-3) s(-1) were measured at pH 8.6. Trace amount of hydrogen peroxide (2x10(-7)-1.0x10(-5) mol l(-1)) was determined in aqueous solution by using the cleavage of Eriochrome black T catalyzed by peroxidase. The method is simple and practical, with high sensitivity and enzymatic activity.