Wang Sheng-bo
2008
Citations
0
Influential Citations
0
Citations
Journal
Lishizhen Medicine and Materia Medica Research
Abstract
Objective To determine Homomangiferin in raw medicine of Mangiferin by HPLC.Methods Homomangiferin was separated on Hypersil ODS 5μm column(4.6 mmID×250 mm) with a mobile phase of methanol-0.1% phosphoric acid solution(32∶68) and detected at a wavelength of 258 nm.Results The calibration curve was linear in the range of 0.042 4~0.848μg(r=0.999 9).The average recovery rate was 97.8% and RSD=2.2%(n=6).Conclusion The method is simple,sensitive and precise.It can be used for the determination of Homomangiferin in raw medicine of Mangifierin.