Zheng Xiao-hui
2009
Citations
0
Influential Citations
2
Citations
Journal
Journal of Xi'an Jiaotong University
Abstract
Objective To establish a new method for the determination of isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate(IDHP) in plasma and tissue in rabbits by high performance liquid chromatography-electrospray ion-trap mass(HPLC-ESI-MSn).Methods Plasma and tissue samples were pretreated by liquid-liquid method,methyl 4-hydroxybenzoate was used as the internal standard,and the multi-reaction mode was applied to determine the concentration of IDHP in plasma and tissue samples.Results The detecting limits of IDHP in plasma,heart,liver,spleen,lung,kidney and brain were 0.54,1.08,1.20,1.62,2.15,1.12 and 1.36ng/mL,respectively;the linear ranges were 0.20-400.0,0.10-200.0,0.01-100.0,0.02-400.0,0.20-800.0,0.01-100.0 and 0.05-100.0μg/mL,respectively.The relative deviations for inter-and intra-day precision were all less than 10%;the average recoveries of the proposed method were within the range of 89.5% to 107.4%.Conclusion The proposed method has the merits of being sensitive and specific,and can be applied in investigating pre-clinical and clinical pharmacokinetics of IDHP.