W. Ai-dong
2012
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0
Influential Citations
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Journal
Journal of Chinese Mass Spectrometry Society
Abstract
Ivabradine and N-desmethylivabradine in human plasma was determined by high performance liquid chromatography coupled with tandem mass spectrometry(HPLC-MS/MS).Diazepam was used as internal standard.Ivabradine and N-desmethylivabradine was separated on a Diamosil C18 column(150 mm×4.6 mm×5 μm).Electrospray ionization(ESI) source was applied,and multiple reaction monitoring(MRM) mode was operated in the positive mode with the monitor ions at m/z 469.2→177.1 for ivabradine,m/z 455.2→177.1 for N-desmethylivabradine and m/z 285.1→193.1 for the interna1 standard,respectively.The linear calibration curve are obtained over the concentration range of 0.101 3—101.3 μg/L for ivabradine and 0.085—25.5 μg/L for N-desmethylivabradine.The limit of quantitation are 0.101 3 μg/L and 0.085 μg/L for ivabradine and N-desmethylivabradine,respectively.The inter-day and intra-day precision(RSD) is less than 15%.The average recoveries are above 75%.