J. Leslie, D. L. Williams, G. Gorin
Mar 1, 1962
Citations
0
Influential Citations
50
Citations
Journal
Analytical biochemistry
Abstract
Abstract N-Ethylmaleimide has been found suitable for the determination of mercapto groups in proteins. Its application to ovalbumin, β-lactoglobulin, and bovine serum albumin has given results in accordance with those of other analytical methods. The first two proteins must be denatured, and different denaturants vary in their action. Since the reactivity of mercapto groups in proteins is determined by many factors, more than one reagent and a single set of conditions should in general be used.