Lincheng Jian
2005
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Journal
The Chinese Journal of Clinical Pharmacology
Abstract
Objective To develop a LC/MS method for quantifying moraci-zine in healthy human plasma. Methods LC:Use Kromasil C18 (150 mm× 4. 6 mm,5μm) with the mobile phase consisting of acetonitrile-10 mmol· L-1 acetateamine buffer - glacial acetic acid (50:50:0. 45 ) . MS: Equipped with an atmospheric pressure chemical ionization (APCI) source was used as detector and was operated in positive ion mode selected reaction monitoring (SRM) mode with the transitions of m/z 427. 9 and m/z 326. 9 used as quantify moricizine and clozapine, respectively. Results the calibration curve was linear over the concentration range of 10. 00-4 000 ng· mL-1, the extracted recovery was (72. 74 ± 3. 61)% . Within-day and between - day precise expressed by relative standard derivation (RSD) less than 15%. The main pharmarcokinetic parameters were follows:tmax was (1.46±0.38)h;Cmax was (550. 8 ± 279. 3) ng·mL-1;t1/2 was (2.41 ± 0.92)h, AUC0-t and AUC0-∞ were (1529. 4 ± 726. 7 )ng·h·mL-1 and (1585. 3 ±740. 7) ng·h·mL-1 respectively by using trapezoid method. Conclusion The assay method is shown to be sensitive, fast, suitable and accurate for determination of moricizine in human plasma.